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Abstract This study evaluates the impact of assimilating precipitable water vapor (PWV) within an observing system simulation experiment (OSSE) framework to improve forecasts of monsoonal mesoscale convective systems (MCSs) in Arizona. Two contrasting case studies differing in convective forcing, longevity, intensity, and coverage are analyzed using a 40‐member ensemble of 1.8‐km resolution Weather Research and Forecasting (WRF) convective‐permitting model (CPM) simulations including the Data Assimilation Research Testbed (DART) system. Synthetic PWV data are derived from a nature run (NR) and bias corrected using real GPS‐derived PWV observations from a campaign during the North American monsoon (NAM) season 2021. These synthetic PWV are assimilated in an inferior model simulation called the control run (CR) to avoid the identical twin problem. Horizontal GPS station spacing experiments (e.g., superobbed, 50 km, 100 km, and 200 km) are conducted to identify configurations that maximize forecast skills. Assimilating the synthetic PWV reduces mean errors (∼2 mm) and dry bias during the first 4–6 hr of the predictions using analyses improved with PWV data assimilation. The 100‐km GPS network optimally captures convective precipitation patterns, outperforming coarser (200‐km) and finer (50‐km) grids due to an improved representation of moisture and winds afforded by PWV data assimilation at the appropriate scales. Topography strongly influences moisture distribution, with elevation‐dependent biases, overestimation in low elevations (0–500 m), underestimation in midelevations (500–2,000 m), and systematic high‐elevation (>2,000 m) biases due to vertically integrated PWV constraints. This study provides actionable insights for optimizing GPS network design and improving convective‐scale modeling in arid/semiarid regions. 

Abstract Mobile element insertions (MEIs) are repetitive genomic sequences that contribute to genetic variation and can lead to genetic disorders. Targeted and whole-genome approaches using short-read sequencing have been developed to identify reference and non-reference MEIs; however, the read length hampers detection of these elements in complex genomic regions. Here, we pair Cas9-targeted nanopore sequencing with computational methodologies to capture active MEIs in human genomes. We demonstrate parallel enrichment for distinct classes of MEIs, averaging 44% of reads on-targeted signals and exhibiting a 13.4-54x enrichment over whole-genome approaches. We show an individual flow cell can recover most MEIs (97% L1Hs, 93% Alu Yb, 51% Alu Ya, 99% SVA_F, and 65% SVA_E). We identify seventeen non-reference MEIs in GM12878 overlooked by modern, long-read analysis pipelines, primarily in repetitive genomic regions. This work introduces the utility of nanopore sequencing for MEI enrichment and lays the foundation for rapid discovery of elusive, repetitive genetic elements. 

Abstract Consensus on the cause of recent midlatitude circulation changes toward a wavier manner in the Northern Hemisphere has not been reached, albeit a number of studies collectively suggest that this phenomenon is driven by global warming and associated Arctic amplification. Here, through a fingerprint analysis of various global simulations and a tropical heating-imposed experiment, we suggest that the suppression of tropical convection along the Inter Tropical Convergence Zone induced by sea surface temperature (SST) cooling trends over the tropical Eastern Pacific contributed to the increased summertime midlatitude waviness in the past 40 years through the generation of a Rossby-wave-train propagating within the jet waveguide and the reduced north-south temperature gradient. This perspective indicates less of an influence from the Arctic amplification on the observed mid-latitude wave amplification than what was previously estimated. This study also emphasizes the need to better predict the tropical Pacific SST variability in order to project the summer jet waviness and consequent weather extremes.